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Seth Meyers was delivering his monologue jokes on Monday night when he delivered one that was met with almost complete silence from the crowd. There was so much silence that it made Meyers break into laughter, rising from his desk to walk off the joke's quick death. He even delightfully acknowledged that he should've known better:

I've been doing this job too long to think that joke would work.

The joke begins at the 3:45 mark: 

http://www.hulu.com/watch/903231
Sources: Vulture | Hulu